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| WBPicture0000008306 | Description | Figure 6. Co-localization of native CPI-2a and Ce-CPZ-1 proteins. Embryo and whole mount fixation were performed as described under Experimental Procedures. CPI-2a was labeled with fluorescein isothiocyanate-conjugated goat anti-rabbit, whereas CPZ-1 was labeled with Texas Red-conjugated goat anti-mouse secondary antibodies. CPI-2a (a) was localized in both germ cells (arrow) and oocytes (lines), whereas CPZ-1 (b) was found in developed oocytes (lines); however, CPZ-1 staining was very faint in germ cells (arrow). CPI-2a and CPZ-1 co-localized (c) in developing oocytes (line) and to some extent also to the germ cells. During ecdysis (d) of the old cuticle, both CPI-2a (e) and CPZ-1 (f) are present at the same regions in the cuticles (g) of the molting worm (arrow). However, whereas CPI-2a was present along the ecdysed cuticle, the CPZ1 enzyme was expressed in only a part of the ecdysed cuticle (g). Localization of CPI-2a and CPZ-1 proteins was observed under a Zeiss fluorescent microscope using fluorescein/isothiocyanate filter sets (magnification, x400). | |||
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| Name | F6.large.jpg | ||||
| Depict | Expr_pattern | Expr7868 | |||
| Expr7869 | |||||
| Anatomy | WBbt:0005843 | ||||
| WBbt:0006796 | |||||
| WBbt:0006797 | |||||
| WBbt:0007028 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2006 | ||||
| Article_URL | DOI | id | 10.1074/jbc.M600254200 | ||
| Journal_URL | TheJournalofBiologicalChemistry | ||||
| Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
| Reference | WBPaper00027761 |
