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WBPicture0000008652 Description: Figure 1. Localization of LET-23 RTK in vulval epithelial cells. Vulval precursor cells are stained with anti-LET-23 antibodies (green) and a cell junction mAb marker (MH27, red). (A) let-23(sy5) rol-6(e187); lin-1(sy254) mutant shows no specific LET-23 staining. (B) Lateral view of an early L2 wild-type hermaphrodite. Strong LET-23 staining is observed in the apical membrane domain, which appears as a thin line along the ventral surface. Weak LET-23 staining is observed in the basolateral membrane domain, located dorsal and anterior to the cell junctions. At this stage, the cell junctions are small, separate rings that appear as lines when seen on edge. (C) Lateral view of a late L2-early L3 wild-type hermaphrodite. LET-23 staining appears in the baso- lateral membrane domains of the vulval precursor cells. Cell junctions have elongated along the anterior-posterior axis, such that the junction from one vulval precursor cell connects with the junctions from adjacent vulval precursor cells. (D and E) Optical cross-sections of vulval precursor cells P6.p (D) and P8.p (E) from a late L2-early L3 wild-type hermaphrodite. The basolateral membrane domain is above the cell junctions, and the apical membrane domain is between or slightly below the cell junctions. LET-23 appears pre- dominantly basolateral in P8.p and both basolateral and apical in P6.p. Out- lines of the vulval precursor cells depicted in C and of the cross-sectional areas depicted in D and E are shown. Cross-sections were generated by rotating three-dimensional images 90 degrees about the dorsal-ventral axis and projecting 1 um along the anterior-posterior axis. (F) Lateral view of an L3 wild-type hermaphrodite, showing granddaughter cells of P6.p. LET-23 staining ap- pears in both the apical and basolateral membrane domains. Dorsal is up and anterior is left, except for D and E. Bars, 10 um (A-C and F); 2 um (D and E).
  Name: F1.large.jpg
  Depict: Expr_pattern: Expr1281
    Anatomy: WBbt:0008112
      WBbt:0008117
      WBbt:0008121
      WBbt:0008125
      WBbt:0008129
      WBbt:0008133
    Cellular_component: GO:0016323
      GO:0016324
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1999
    Article_URL: DOI id 10.1091/mbc.10.6.2087
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00003566