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| WBPicture0000008801 | Description | Figure 5. HAF-6 is expressed in intestine and germline and localizes to intracellular, reticular membranes. Endogenous GFP fluorescence from a rescuing HAF-6::GFP fusion protein (Figure 4, plasmid pLT531) and immunolocalizations using anti-HAF-6 and control antibodies were used to investigate subcellular distribution patterns. (A) HAF-6::GFP fluorescence patterns in live animals (left and center) are similar to the patterns observed in fixed animals decorated with an anti-HAF-6 antibody (right). White arrows, intestinal nuclei; red arrows, autofluorescent granules. (B) The HAF-6::GFP protein (left) is localized to the same compartment as calreticulin (CRT-1), a primarily ER resident with a C-terminal KDEL sequence. Similar results were obtained in cohybridization experiments using the anti-HAF-6 antibody with anti-CRT-1. HAF-6 is a transmembrane protein, and 100% spectral overlap with CRT-1 is not anticipated; nonetheless, co-compartmentalizaion is observed. (C) Cohybridization experiments were performed in transgenic animals expressing both endogenous HAF-6 and a HAF-6::GFP fusion protein. The signals from anti-GFP and anti-HAF-6 overlapped, indicating that the fusion protein is expressed in all the compartments where the endogenous protein is located. Confocal images were captured using a Zeiss LSM 510 META. Bars, 5 um on all panels except A (10 um). | |||
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| Name | F5.large_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000008800 | |||
| Depict | Expr_pattern | Expr4794 | |||
| Expr4795 | |||||
| Anatomy | WBbt:0005772 | ||||
| WBbt:0005784 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2006 | ||||
| Article_URL | DOI | id | 10.1091/mbc.E06-03-0192 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00027644 |
