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WBPicture0000008856 | Description | Figure 3. An antibody specific for DAF-19A/B detects DAF-19 in all nonciliated neurons. (A) DAF-19 epitopes recognized by two different antibodies. Antibody AbDAF19N is specific for the long isoforms DAF-19A/B, whereas antibody AbDAF19C recognizes all three isoforms DAF-19A/B/C. (B-G) gfp reporter lines stained with antibodies against DAF-19. AbDAF19N detects DAF-19A/B only in nonciliated neurons (D and E) and not in ciliated sensory neurons (csn) (B and C). Ciliated sensory neurons express only DAF-19C. Because ciliated sensory neurons do not express DAF-19A/B, DAF-19C can be visualized by AbDAF19C (F and G). ceh-23::gfp marks ciliated sensory neurons, tph-1::gfp marks the serotonergic neuron ADF (ciliated), and nmr-1::gfp marks interneurons (nonciliated); see Supplemental Table 1 for details. (H) Schematic summary of all neurons investigated in the head region (blue, AbDAF19N; yellow, AbDAF19C; white, not determined neurons; and gray, no DAF-19 expression detected). | |||
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2008 | ||||
Article_URL | DOI | id | 10.1091/mbc.E08-04-0416 | ||
Journal_URL | MolecularBiologyoftheCell | ||||
Publisher_URL | TheAmericanSocietyForCellBiology | ||||
Reference | WBPaper00032252 |