WormBase Tree Display for Picture: WBPicture0000008986

WBPicture0000008986 Description: Figure 1. GFP-BAF-1 protein expression. (A) Schematic view of the baf-1- gfp construct used for generating transgenic strains. It includes the baf-1 promoter driving the gfp gene fused to the baf-1 ORF followed by the baf-1 3' untranslated region. (B-D) A stronger GFP-BAF-1 signal was detected at the nuclear periphery, whereas weaker signals were detected in the nucleoplasm and cytoplasm. GFP-BAF-1 was detected in the gonad (arrowhead in B) in young embryos (arrows in B) and in late embryos (not depicted), in L1 larvae (C), and in adult worms (arrow in D points toward the vulva). (E and F) DIC, left; GFP fluorescence, right. Although GFP-BAF-1 was localized normally at the nuclear periphery of control embryos (L4440; E), it was mislocalized in lmn-1(RNAi) embryos (arrowheads in F), where the GFP-BAF-1 was also found associated with anaphase chromatin bridges (arrow in F). The image in B was obtained with a confocal microscope, whereas images in C-F were viewed with an Axioplan II microscope (Carl Zeiss MicroImaging, Inc.). Bars, 10 um.
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  Acknowledgment (5)
  Reference: WBPaper00030942