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| WBPicture0000009432 | Description | Figure 2. The PBO-4 Na+/H+ Exchanger Is Expressed on the Surface of the Intestine. (A) Confocal images of transgenic animals expressing the pHluorin::PBO-4 fusion protein. DIC (top), confocal slice (middle), and confocal projection (bottom) of an adult hermaphrodite expressing the GFP fusion protein. Fluorescence was observed on the basolateral surface of the posterior intestine cells only. Animals carrying this fusion protein were rescued for posterior body contractions (pbo-4(ok583); oxEx584), demonstrating that the fusion protein is functional and properly localized. The dashed lines indicate the intestinal boundary in the DIC image. Scale bar is 50 μM.(B) Top panel shows cross-sectional electron micrograph of the posterior end of a wild-type C. elegans adult hermaphrodite (top). The intestine (green), muscle (red), and epidermis (blue) are pseudocolored, and the region of interest is denoted by a white box. Scale bar is 1 μm. Bottom panel shows closeup of the region of interest. Note the muscle extends a process to form a long muscle-intestinal interface (arrowhead). Scale bar is 0.5 μm. | |||
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| Name | F2.jpg | ||||
| Depict | Expr_pattern | Expr7877 | |||
| Anatomy | WBbt:0005772 | ||||
| Cellular_component | GO:0016323 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2008 | ||||
| Article_URL | DOI | id | 10.1016/j.cell.2007.10.058 | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00031426 |
