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WBPicture0000009519 | Description | Figure 4. Expression Pattern of vab-1 Reporter Constructs. Confocal images of VAB-1::GFP expression from the juIs24 transgene (visualized using anti-GFP antibodies [green]) and epidermal cell junctions (MH27 antigen [red]). Bars, 15 μ m. (A) VAB-1::GFP expression in postgastrulation embryo (200-250 min). Ventral view. Not all VAB-1-expressing cells have been identified at this stage; based on position most of the cells are neuroblasts. Some epidermal precursors may also express VAB-1::GFP. (B and C) Beginning of ventral closure, ventrolateral and ventral views (D). Later in ventral closure, leading epidermal cells are marked (arrows). (E) Midventral closure; leading cells have met at ventral midline, ventral view; ventral pocket has not yet closed. (F) Completed ventral enclosure (comma stage). (G) L1 larval animal showing expression of VAB-1::GFP ( juIs33 transgene) in the nervous system; nerve ring and ventral nerve cord are arrowed. Strong expression is also seen in the procorpus and terminal bulb of the pharynx. (H) Expression of VAB-1::GFP in axons in late larval animal; lateral view, close-up of expression in D neuron axon commissure (arrowed) and ventral cord. |
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Name | F4.jpg | |
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Reference | WBPaper00003020 |