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WBPicture0000009520DescriptionFigure 4. UNC-73 Expression Pattern. (A) At the beginning of neurulation (comma stage embryo), expression of unc-73/gfp is strongest in the region of the developing nervous system in the head (white arrowheads) and in the ventral nerve cord (yellow arrows).(B) The P cells (white arrowheads) begin to express in early L1 just before they intercalate on the ventral midline. The process of intercalation proceeds from anterior to posterior (left to right) and is paralleled by a progressive anterior to posterior increase in expression of the unc-73/gfp reporter.(C and D) In mid-L1, expression comes on in the Q neuroblasts on the left and right (white arrowheads in C and D, respectively). In this animal, QL has migrated posteriorly and has yet to divide, and QR has migrated anteriorly and divided once.(E) The honeycomb expression in this L4 animal is most likely the somatic gonad. The distal tip cell (yellow arrow) at the leading edge of the growing somatic gonad and the excretory canal (thick blue arrows) also express unc-73/gfp. The dorsal nerve cord (white arrowheads) is also visible. Red arrows in (B), (C), and (F) point to CAN neurons. The transgenes in the animals of (A) and (F) were extrachromosomal and may have been lost in some cells.(F) The motorneurons in the ventral nerve cord (e.g., white arrowheads) express throughout postembryonic development. The HSNL neuron (thick yellow arrow) and spindle-shaped body muscles are also visible.(G) A wild-type L3 animal stained with an affinity purified polyclonal antibody to UNC-73. Note intense staining of nerve ring (yellow arrow) and ventral nerve cord (white arrowheads).Scale bars = 10 μm.
NameF4.jpg
DepictExpr_patternExpr1438
Anatomy (13)
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1998
Article_URLDOIid10.1016/S0092-8674(00)81406-3
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00003027