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| WBPicture0000009524 | Description | Figure 5. CED-7 Protein Expression. (A) Western blot analysis of CED-7 protein. We used affinity-purified anti-CED-7 antibodies to probe a blot of embryonic extracts from wild-type, ced-7(n1996), and ced-7(n2094) animals. Protein extracts (200 μg) were loaded in each lane. That the equal amount of protein was loaded in each lane was confirmed by Ponceau S staining (data not shown). The sizes of molecular weight markers (High range, Bio-Rad) and the position of the CED-7 protein are indicated.(B) Anti-CED-7 antibody staining of a wild-type embryo at approximately the 50-cell stage (left) and a wild-type embryo at 558-cell stage (right). The two brightly stained cells on the ventral midbody of the right embryo are Z2 and Z3, the germline precursor cells. The bar represents 10 μm; the same scale applies to (C)-(F).(C) Anti-CED-7 antibody staining of a ced-7(n1996) mutant embryo at approximately the 50-cell stage, prepared in parallel to (B) and lacking any specific staining.(D) Anti-CED-7 antibody staining of the head of a wild-type L1 (first larval stage) hermaphrodite. The pharyngeal-intestinal valve and the cell bodies of the two amphid sheath cells are indicated by open and closed arrows, respectively. Anterior is to the left. This picture is a projection of four serial confocal images.(E) Anti-CED-7 antibody staining of the tail of a wild-type L1 hermaphrodite. The two phasmid sheath cells are indicated by arrows. Anterior is to the left.(F) Anti-CED-7 antibody staining of the germline in part of the anterior gonadal arm of a wild-type adult hermaphrodite. | |||
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| Depict | Expr_pattern | Expr1426 | |||
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| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 1998 | ||||
| Article_URL | DOI | id | 10.1016/S0092-8674(00)81201-5 | ||
| Journal_URL | Cell | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003091 |
