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WBPicture0000009553 Description: Figure 3. Complex of CHN-1, UFD-2, and UNC-45(A-C) Pull-down experiments with recombinant proteins followed by Western blot analysis and Coomassie blue staining. (A) Interaction of UFD-2 and UNC-45 in vitro. GST-UFD-2 was incubated with purified FLAG-tagged UNC-45 (UNC-45FLAG) and pulled down with glutathione-Sepharose beads. GST alone with UNC-45FLAG served as the negative control. (B) In vitro binding of UNC-45 by UFD-2 or CHN-1. Equimolar amounts of GST-UFD-2 and GST-CHN-1 were incubated with the same amount of purified FLAG-tagged UNC-45 (UNC-45FLAG) and pulled down with glutathione-Sepharose beads. GST alone with UNC-45FLAG served as the negative control. (C) Complex of CHN-1, UFD-2, and UNC-45. GST-UFD-2 was incubated with purified FLAG-tagged UNC-45 (UNC-45FLAG) and myc-tagged CHN-1 (mycCHN-1) together, mycCHN-1, or UNC-45FLAG alone and pulled down with glutathione-Sepharose beads. GST alone with UNC-45FLAG and mycCHN-1 served as the negative control.(D-H) Transgenic expression of chn-1 and ufd-2 in muscle cells of N2 wild-type animals. (D) Two-fold embryo expressing GFP::chn-1 in the cytoplasm of most cells. (E) L4 larva expressing GFP::chn-1 in pharynx muscles. (F) L2 larva showing expression of GFP::chn-1 in body wall muscle cells (arrows). Inset shows detailed view of the subcellular localization: GFP::chn-1 is expressed mainly in the cytoplasm and not in the nucleus of somatic muscle cells (arrows pointing to the nucleus). (G) ufd-2::GFP is expressed in pharyngeal muscles of a L2 larva. (H) L4 larva expressing ufd-2::GFP in the cytoplasm and the nucleus of body wall muscle cells (arrow points to the nucleus of a body wall muscle cell). Scale bars, 10 μm.
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  Crop: Crop_picture: WBPicture0000009554
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1016/j.cell.2004.07.014
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00024334