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WBPicture0000009585 | Description | Figure 4. Expression Analysis of the ttx-3 Gene. (A) Expression pattern analysis of ttx-3 in wild-type animals after hatching. The schematic drawing of the AIY interneuron is adapted from White, et al. 1986.(B) Embryonic expression pattern of ttx-3. The white arrow points to the AIY cells in late embryos. We believe the additional eight staining cells in the embryos to be head muscles, due to their characteristic morphology at late embryonic stages. Expression of ttx-3 in these muscles cells is highly dynamic and developmentally regulated: in 1.5- and 2-fold embryos, the complete set of ten cells stains consistently for GFP, while in embryos just before hatching, only 47% of animals show muscle staining in addition to AIY staining (as shown in the left photograph; the remaining 53% of animals show staining in AIY only; sample size, 106 animals). Muscle staining perdures but further decreases throughout early larval stages (6% of animals show muscle staining + AIY staining; 94% show AIY staining only; sample size, 279 animals), while in late stage larvae and adults, ttx-3 expression is exclusively confined to the pair of AIY interneurons. We cannot rule out that the dynamic expression pattern in muscle reflects a lack of a promoter element in our ttx-3-GFP expression construct. | |||
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Name | F4.jpg | ||||
Depict | Expr_pattern | Expr1464 | |||
Anatomy | WBbt:0005413 | ||||
WBbt:0006761 | |||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 1997 | ||||
Article_URL | DOI | id | 10.1016/S0896-6273(00)80944-7 | ||
Journal_URL | Neuron | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00002876 |