WormBase Tree Display for Picture: WBPicture0000009631
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WBPicture0000009631 | Description | Figure 3. Expression of C. elegans Endophilin. (A) Endophilin is expressed in neurons. 4.5 kb of promoter sequence upstream of unc-57/T04D1.3 was fused to GFP. A confocal fluorescence micrograph of an adult hermaphrodite is shown. The worm is positioned so that the ventral cord is exposed; the anterior of the worm is left. Expression is observed in all neurons. Abbreviations: NR, nerve ring; VNC, ventral nerve cord; CB, cell bodies; PI, posterior intestine. Neuromuscular junctions are found along the ventral nerve cord.(B) Endophilin is localized to synapses. Dorsal view of the head of an adult hermaphrodite; anterior is left. Top, GFP-tagged endophilin (green). This tagged construct rescues the unc-57 phenotype. Bracket: punctate staining is observed at the neuromuscular junctions of the SAB neurons. Bright staining to the right is a cross-section through the nerve ring (NR), the major neuropil in the head. Middle, synaptobrevin immunoreactivity (red) is localized to synaptic regions. Bottom, merged image demonstrates that endophilin-GFP colocalizes with synaptobrevin at synapses. Antibody staining was performed as previously described (Miller and Shakes, 1995).(C) Endophilin is localized to neuromuscular junctions. A section of the dorsal and ventral nerve cords of a worm expressing GFP-tagged endophilin (genotype, lin-15(n765ts); oxIs91[UNC-57-GFP; lin-15+]). The ventral and dorsal cords show punctate localization of the GFP-tagged endophilin protein (small arrows), consistent with a localization to neuromuscular junctions. Cell bodies of the motor neurons are only located in the ventral nerve cord (arrowheads).(D) Endophilin is mislocalized in a kinesin mutant. GFP-tagged endophilin remains in the motor neuron cell bodies in the absence of the synaptic vesicle kinesin (unc-104(e1265); oxIs91[UNC-57-GFP; lin-15+]). | |
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Name | F3_A.jpg | ||
Crop | Cropped_from | WBPicture0000009630 | |
Depict | Expr_pattern | Expr2769 | |
Expr2770 | |||
Anatomy | WBbt:0003679 | ||
WBbt:0005829 | |||
WBbt:0006749 | |||
Cellular_component | GO:0008021 | ||
Acknowledgment (5) | |||
Reference | WBPaper00006213 |