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| WBPicture0000009641 | Description | MEC-1 Localization. (A) Expression of GFP from a mec-1 promoter. The six touch neuron cell bodies are indicated. The left side is seen on the upper worm, the right side on the lower worm. Scale bars here and in panels (B)-(E), 50 μm; scale bars in subsequent panels, 10 μm. (B-E) β-galactosidase expression from mec-1::lacZ fusions. Fusions with MEC-1(1-22) were used in (B) and (C); fusions with MEC-1(1-45) were used in (D) and (E). The fusions in the second panel of each pair contained an artificial transmembrane domain. (F-H) MEC-1::GFP fluorescence in (F) the cell bodies of PLM, PLN, and ALN neurons showing perinuclear expression (presumably in the endoplasmic reticulum), (G) a PLM touch neuron, and (H) an ALM touch neuron. Puncta (white dots) are seen above a more uniform fluorescence. Fluorescence is never seen in the processes of the non-touch receptor neurons. (I) GFP fluorescence from a mec-1 promoter fusion in an ALM touch neuron. (J) MEC-1::GFP fluorescence on a PVM ventral process. The uniform fluorescence seen on the lateral processes is not found. Lines perpendicular to the PVM process are the annuli, which we find autofluoresce in the GFP and YFP channels of the confocal microscope. The confocal image has been collapsed from a Z series to show the annuli. (K) GFP fluorescence from a mec-1 promoter fusion in the ventral process of a PVM touch neuron. | |||
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| Name | F3_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000009640 | |||
| Depict | Expr_pattern | Expr3463 | |||
| Anatomy | WBbt:0003832 | ||||
| WBbt:0003939 | |||||
| WBbt:0003953 | |||||
| WBbt:0003954 | |||||
| WBbt:0004102 | |||||
| WBbt:0004103 | |||||
| WBbt:0004104 | |||||
| WBbt:0005237 | |||||
| WBbt:0006851 | |||||
| WBbt:0006855 | |||||
| Cellular_component | GO:0005783 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1016/j.neuron.2004.11.010 | ||
| Journal_URL | Neuron | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00024622 |
