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WBPicture0000009729 Description: Figure 2. The C. elegans Sm Proteins Localize to P Granules. (A and B) Meiotic pachytene germ cells of the adult germline stained with the anti-Sm Y12 monoclonal antibody (A) and PGL-1 (B). DAPI staining of nuclei is also shown.(C and D) Four-cell and eight-cell embryos stained with Sm human sera (C) and PGL-1 (D). Merged images show that PGL-1 and Sm staining are coincident within P granules (data not shown). The Sm monoclonals KSm4 and 7.13, as well as an SmG polyclonal, also stained P granules (Supplementary Material).(E-H) Embryos of approximately 50 cells were stained with Sm human sera (E, G) or PGL-1 (F, H). The embryo in (G) and (H) was from an SmE,D1,D3 (RNAi) animal, while those in (E) and (F) are from a noninjected control animal. The exposures in (E) and (G) are equivalent. Quantitation of the reduction of Sm staining by Sm depletion with several Sm antibodies is presented in the Supplementary Material (Table S2). PGL-1 particles are detected in four cells in (H).The scale bars are 2.5 μm (A, B), 10 μm (C, D), and 5 μm (E-H).
  Name: F2.jpg
  Depict: Expr_pattern: Expr2229
    Cellular_component: GO:0043186
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1016/S0960-9822(02)01111-9
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00005457