WormBase Tree Display for Picture: WBPicture0000009794

WBPicture0000009794 Description: Figure 4. Dynamics of EFF-1::GFP Localization. (A) Ventral accumulation between two migrating cells begins just after cell contacts are made.(B) Dorsal accumulation occurs between two cells with preexisting cell contacts. Yellow arrows indicate EFF-1::GFP accumulation. Anterior is at the top, and posterior is at the bottom of each panel. Approximate time of development is shown.(C) Quantification of plasma-membrane-localized EFF-1::GFP. Inset, profiles of fluorescence intensity across the cytoplasm and contact interface of neighboring cells are shown for a single contact. The time of each profile is indicated by a color-matched underline on the time axis of the main plot. Main plot, separate measurements of both dorsal and ventral fusion contacts were taken for each of four embryos. Localized (within the peak) and background values (regions outside peak) from the four embryos were normalized, averaged, and plotted versus time (see Experimental Procedures). Error bars show standard deviation. R2 values for linear fits are 0.976 for ventral and 0.918 for dorsal accumulation versus time.(D) Heat-shock-induced EFF-1::GFP expression in an early embryo. Localization occurs only between cells expressing bright EFF-1::GFP. Yellow arrow indicates localization along cell contact. Scale bar, 10 μm.
  Name: F4.jpg
  Depict: Expr_pattern: Expr3230
    Cellular_component: GO:0016324
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2005
    Article_URL: DOI id 10.1016/j.cub.2005.01.054
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00025022