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WBPicture0000009831 Description: Figure 1. The C. elegans Midline Is Composed of a Closely Aligned Row of Motoneuron Cell Bodies, Termed eMNs. (A) Representative EM cross-section of a developing embryo, highlighting the spatial relationship of eMNs and VNC axons. In all analyzed cross-sections, AVG and PVQ axons are found in immediate contact with eMN cell bodies. PVP axons are more exterior and in contact with AVG and PVQ axons. eMN cell bodies generally straddle the midline at the junction of bilaterally symmetric pairs of P cells. Electron micrographs have been kindly provided by R.M. Durbin. More examples can be found in Figure S1.(B) Schematic drawing of the embryonic VNC adapted from [11]. Gray cells at the midline are individual DA class eMNs (DA1, DA2, etc.), red cells are DB class eMNs (DB1, DB2, etc.), and blue cells are DD class eMNs (DD1, DD2, etc.).(C) A post-2-fold stage embryo, showing eMNs labeled with two different gfp transgenes juIs76 (unc-25::gfp) and evIs82b (unc-129::gfp). Still being in the eggshell, the animal is strongly twisted. Compare to (D).(D) wrk-1 expression pattern in a post-2-fold-stage embryo (twisted due to being in the eggshell). wrk-1 expression was determined by means of a reporter construct (wrk1::TM::gfp), in which 4 kb of sequences upstream of the ATG start codon, all exons, and the first three introns of the wrk-1 locus were fused in frame to gfp (see Figure S2 for all reporter constructs used). This construct is able to rescue the phenotype of wrk-1 mutant animals (see Figure 3A), thereby demonstrating that it reveals expression in functionally relevant cells. In the embryonic nervous system, expression was observed in a limited set of neuron types, including all eMNs (DA, DB, and DD; [C]). Possibly due to transgene properties, the construct is not always expressed at the same levels in each eMN. Expression in eMNs is observed during embryonic stages and fades during larval stages. The expression of wrk-1 reporter gene fusions in eMN neurons has been independently confirmed by a microarray-based profiling of motoneuron-expressed genes [31]. Expression of wrk-1::TM::gfp is also seen in some other unidentified embryonic cells, which likely correspond to neurons that express wrk-1 postembryonically (see Figure S2 for more details).
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  Crop: Crop_picture: WBPicture0000009832
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2006
    Article_URL: DOI id 10.1016/j.cub.2006.08.056
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00028566