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WBPicture0000010106 | Description | Fig. 2. Expression pattern of ubc-25::gfp in C. elegans. (A) UBC-25::GFP fluorescence in an adult hermaphrodite. The highest fluorescence intensity is found in the pharynx (left arrow), a muscular organ that serves in the intake of food. Additional fluorescence is detected in the vulval muscles (middle arrow), in the anal muscles (right arrow), and in the two lateral nerve chords. (B) A close-up at the tail region of a hermaphrodite with a predominant neuronal UBC-25::GFP expression. GFP fluorescence is visible in the nerve chords and further neuronal projections as well as in neuronal cell bodies (arrow). A gamma factor of 2.4 was used to improve the visibility of fluorescence in the nerve connections. This procedure also amplifies background fluorescence. (C) The head region of an adult hermaphrodite with a predominantly neuronal UBC-25::GFP expression. UBC-25::GFP fluorescence is present in neuronal cell bodies surrounding the pharynx muscle. (D) A micrograph of the vulva region of a different hermaphrodite, in which the nerve chords and the neurons innervating the egg laying apparatus fluoresce (arrow). (E) Cytoplasmic and nuclear ubc-25::gfp expression in the body wall muscles of a L2 stage larva (arrow). (F) ubc-25::gfp expression in a comma stage embryo (anterior left, dorsal at top, a lateral left optical plane is shown). Arrows point to D lineage muscle precursor cells. The white circled area corresponds to anterior nerve cells. The cells above the white line are muscle precursor cells from the D- and MS-lineages. (G) The corresponding Nomarksi DIC image. The scale bars are 100 um in (A) and 20 um in (B-F). | |||
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Name | F2.jpg | ||||
Depict | Expr_pattern | Expr2563 | |||
Anatomy | WBbt:0003679 | ||||
WBbt:0003681 | |||||
WBbt:0005803 | |||||
WBbt:0005813 | |||||
WBbt:0005821 | |||||
WBbt:0005829 | |||||
WBbt:0006750 | |||||
WBbt:0006769 | |||||
WBbt:0006807 | |||||
WBbt:0006808 | |||||
Cellular_component | GO:0005634 | ||||
GO:0005737 | |||||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 2003 | ||||
Article_URL | DOI | id | 10.1016/S0006-291X(03)00824-6 | ||
Journal_URL | BiochemicalandBiophysicalResearchCommunications | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00005882 |