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WBPicture0000010188DescriptionFigure 4. Tissue expression of CeTMIII isoform with tmy-1::lacZ and tmy-1::gfp transgenes. (a) b-Galactosidase expression induced in pharyngeal muscles and intestinal cells of L4 worm by pTMIIIZ1743. (b) Expression was observed in m1, m3, m4, m5 and m7 muscles of the pharynx. (c) b-galactosidase activity was also induced in germ-line tissue between L2 and L3 stages. At this point, pharyngeal expression was complete and intestinal staining was restricted only to the posteriormost region. (d) Expression was induced only in the pharynx in L1 worm indicating a stage specific expression. (e) and (f) GFP expressions induced in pharynx, intestines and germ-line tissue by pTMGIII1743 in L4 and L3 worms, respectively. In Figure 3 and Figure 4, fixation was performed for five hours for muscle cell identification, whereas other lacZ panels were fixed for ten hours.
NameF4.jpg
DepictExpr_patternExpr1680
AnatomyWBbt:0003681
WBbt:0003721
WBbt:0003737
WBbt:0003739
WBbt:0003740
WBbt:0003744
WBbt:0005772
WBbt:0005784
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2001
Article_URLDOIid10.1006/jmbi.2001.5052
Journal_URLJournalofMolecularBiology
Publisher_URLElsevier
ReferenceWBPaper00004944