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| WBPicture0000010194 | Description | Figure 2. One promoter for Ce titin drives expression of the two largest Ce titin isoforms in muscle. (a)-(d) Fluorescent images of GFP expression driven by 2.3 kb of genomic sequence upstream of the 5' end of the largest mRNAs (CeTPro in Figure 1(a)). This construct also contains the first intron and exonic sequence encoding the first 96 aa of the 2.2 MDa and 1.2 MDa isoforms. (a) Low powered view of whole adult showing expression in body wall muscle cells; scale bar represents 100 um. (b) Expression in enteric muscle; scale bar represents 10 um. (c) Expression in vulval muscle; scale bar represents 10 um. (d) Same animal as in (a), but at higher magnification; scale bar represents 10 um. (e)-(g) High magnification view of CeTPro animals stained with antibodies to vinculin. (e) GFP signal, (f) vinculin signal, and (g) merged signals by confocal microscopy; scale bar (depicted in (e), applies to (e)-(g)) represents 5 um. Thus, the Ce titin 96 amino acid residues-GFP localizes to dense bodies. | |||
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| Name | F2.jpg | ||||
| Depict | Expr_pattern | Expr2286 | |||
| Anatomy | WBbt:0005772 | ||||
| WBbt:0005813 | |||||
| WBbt:0005821 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1016/S0022-2836(02)00970-1 | ||
| Journal_URL | JournalofMolecularBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00005546 |
