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| WBPicture0000010699 | Description | Figure 3. Expression and cell-nonautonomous activity of bam-2. (A) Expression and rescue ability of bam-2 promoter derivatives driving a full-length bam-2 cDNA::gfp fusion gene. bam-2 expression from the VulF vulval cell rescues bam-2 VC neuron-branching defects. (B to D) Expression pattern of bam-2 promoter derivatives (ventral views). (B) bam-2 promoter (~600 bp) exhibits activity in a restricted subset of neurons in the ventral nerve cord and head and tail ganglia. (C) bam-2 upstream sequence (~5 kb) exhibits additional promoter activity in cells of the somatic gonad. The position of the vulva in (B) and (C) is marked by arrowheads. (D) A fusion of a ~2.4-kb distal bam-2 upstream sequence and the 600-bp proximal element exhibits promoter activity in additional longitudinal PLN axon tracts (asterisks) and in the VulF vulval cell. Inset shows an enlarged view of VulF. (E and F) bam-2 is expressed from mid-L4 in VulF (open arrowheads). Lateral views show overlay of VulF GFP fluorescence (delta-xbaIp:bam-2::gfp) (E) on a differential interference contrast image of the same L4 worm (F). (G and H) delta-xbaIp:bam-2::gfp expression in the toroidal-shaped VulF cell [(G), ventral view] and in an adult worm [(H), lateral view]. All images except (E) and (F) are flattened confocal Z-series. Anterior is to the left. Scale bars, 10 um. |
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| Name | F3.large.jpg | |
| Depict (2) | ||
| Acknowledgment (5) | ||
| Reference | WBPaper00006123 |
