WormBase Tree Display for Picture: WBPicture0000010920

WBPicture0000010920 Description: Figure 9. Within the ventral nerve cord, UNC-31 is concentrated presynaptically at or near active zones and largely at cholinergic synapses. (A) UNC-31 immunostaining in the ventral nerve cord. Maximum intensity projections of a z-series through the cord are shown. The four panels are different color combinations of the same image. (Top) Red channel showing UNC-17 staining to mark cholinergic vesicle clusters. (Second channel) Merged UNC-17 (red) and UNC-31 (green) channels showing that UNC-31 is concentrated within cholinergic synapses in the ventral cord. (Third channel) Locations of active zones at cholinergic synapses as indicated by an antibody to UNC-10 (blue). Most of the blue spots not associated with cholinergic synapses likely indicate the locations of noncholinergic synapses in the cord. (Bottom) Merged image of all three channels. (B) Enlargements of clusters of synapses in the ventral cord showing that UNC-31 and UNC-10 often localize to the same subsynaptic region (at or near the active zone). Asterisks indicate synaptic sites having strong UNC-31 staining, but weak or little UNC-10. Arrowheads indicate a few examples of synapses with strong UNC-10 and weak UNC-31. Arrows in the bottom right panel indicate noncholinergic active zones that lack concentrated UNC-31.
  Name: F9.large.jpg
  Depict: Expr_pattern: Expr4798
    Anatomy: WBbt:0005829
    Cellular_component: GO:0048786
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2006
    Article_URL: DOI id 10.1534/genetics.105.049577
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00026941