WormBase Tree Display for Picture: WBPicture0000010990

WBPicture0000010990 Description: Figure 1. Organization of the num-1 locus and expression of num-1A and num-1B. (A) The organization of the exons and introns in num-1 is shown above the scale bar. Boxes represent exons. The lines connecting them show the splicing patterns of the primary transcripts determined by characterization of cDNAs and RT-PCR products. The region spanning parts of exons 3 and 4 of num-1A that encodes the PTB domain is shaded. The regions deleted in num-1(bc365) and num-1(ok433) are indicated by lines below the scale bar. (B) Transgenes used to study num-1 expression and function. Each line is a schematic of part of a plasmid construction used in this study. The plasmid pVB63LN contains an 8.4-kb XhoI-SpeI fragment that spans the entire num-1 locus. The other fragments encode different NUM-1::GFP fusion proteins. In each case, the gfp gene has been inserted immediately prior to the stop codon of num-1. (C-H) Photomicrographs of hermaphrodite worms harboring a num-1A::gfp transgene viewed with either Nomarski differential interference contrast (DIC) (C) or fluorescence (D-J) optics. The worms were viewed from either the left or the right. C-F show medial optical sections; for G, I, and J, the optical section was a plane just below the baso-lateral membrane of the intestine. The images in C-H were obtained with the aid of a Leica DMRB compound microscope. Images in I and J were obtained with the aid of Leica confocal microscope. The worms in C-I were of the genotype unc-4(e120); svIs27 [num-1A::gfp unc-4 (+)]. The svIs27 array harbors the plasmid pVB68LN, which lacks sequences necessary for the expression of num-1B. The worm in J was wild-type N2 control. The arrows in C and D indicate the basal (b) and apical (a) cell membranes of the intestine. The arrowhead in D indicates the position of an adherens junction separating adjacent intestinal cells. The arrow in E indicates the intestine primordium in the embryo. The arrows in F indicate the pharynx (p) and nerve ring (n), respectively. The arrows in G indicate two coelomocytes (c). Note also the punctate pattern within the intestine in this focal plane. The arrow in H points to the adherens junctions separating the apical and basal domains of the seam cells. (I) Confocal micrograph of the intestine of an svIs27 hermaphrodite showing the punctate staining of NUM-1A::GFP close to the baso-lateral membrane of the intestine. (J) A confocal micrograph of a wild-type hermaphrodite taken with identical settings. Bars in C-H are 20 um; in I and J, 4 um. (K-N) Photomicrographs of L4 hermaphrodite worms harboring a num-1B::gfp transgene viewed with either Nomarski DIC (K and M) or fluorescence (L and N) optics. 'can' denotes the excretory canal. The uv1 cells are four neurosecretory cells in the uterus; two of the four are seen in this focal plane. (O) Diagram showing the arrangement of the intestine, which is shaded. At left is shown a medial saggital section viewed from the left. A, anterior; P, posterior; D, dorsal; V, ventral. The baso-lateral and apical membranes of the intestine are indicated. (Right) A transverse section viewed from the anterior. (P) Schematic of C. elegans, Drosophila, and human Numb proteins. All three contain both a PTB domain and at least one DPF motif. Human and Drosophila Numb proteins also harbor the NPF motif.
  Name: F1.large.jpg
  Depict: Expr_pattern: Expr8115
    Anatomy: WBbt:0003681
      WBbt:0005751
      WBbt:0005772
      WBbt:0005775
      WBbt:0006749
      WBbt:0006760
      WBbt:0006791
    Cellular_component: GO:0005886
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2008
    Article_URL: DOI id 10.1534/genetics.108.087247
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00031894