WormBase Tree Display for Picture: WBPicture0000011046

WBPicture0000011046 Description: Figure 2. LIN-56 is broadly expressed and localized to nuclei. (A) Immunoblot of wild-type and lin-56(n2728) protein extracts probed with anti-LIN-56 antibodies. The arrow indicates the band that corresponds to LIN-56. Molecular weights of marker proteins are indicated at left in kilodaltons. (B-E) Whole-mount staining with anti-LIN-56 antibodies (red), DAPI (blue), and either anti-tubulin antibody (green) in embryos or MH27 antibody (green) in larvae as a fixation control. Scale bars, 5 um. Anterior is to the left in all images. pb, polar body. (B and C) LIN-56 staining is observed in the nuclei of most if not all cells throughout embryonic development. Shown are (B) four-cell and (C) late-stage wild-type embryos. (D) Nuclear staining is never observed with anti-LIN-56 antisera in lin-56(n2728) animals. Shown is a four-cell lin-56(n2728) embryo. (E) LIN-56 is present in the P(3-8).p vulval equivalence group and in their descendants during wild-type vulval development. Shown is the midbody of a wild-type L2 larva. Arrowheads point to nuclei of P(3-8).p. MH27 stains the apical borders between the Pn.p cells. (F and G) LIN-56 does not colocalize with chromatin during metaphase or anaphase. F and G show two-cell wild-type embryos. ana, anaphase; meta, metaphase; pro, prophase; telo, telophase.
  Name: F2.large.jpg
  Depict: Expr_pattern: Expr9238
    Cellular_component: GO:0005634
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2011
    Article_URL: DOI id 10.1534/genetics.110.124487
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00037906