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WBPicture0000011195DescriptionDevelopmental profile of fem-3 transcripts. Poly(A)+ RNAs were isolated from developmentally staged hermaphrodites and were separated by electrophoresis in a 2.0% denaturing agarose gel and transferred to nitrocellulose. The filter was hybridized to a fem-3 probe (A) (cDNA clone pJK50, see Materials and methods) and then rehybridized to an act-1 probe (B). The amount of act-1 RNA in total RNA appears to be constant throughout postembryonic development (Krause and Hirsh 1984); therefore, the act-1 signal serves as an internal control for the amount of RNA loaded. Arrows indicate fern-3 transcripts. (A and B) (Lane E) Embryonic RNA; (lanes L1-L4) RNAs from first through fourth larval stages, respectively; (lane A I Adult RNA).
NameFigF.jpg
DepictExpr_patternExpr1382
AcknowledgmentTemplateWormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1988
Article_URLDOIid10.1101/gad.2.5.606
Journal_URLGenes&Development
Publisher_URLColdSpringHarborLabPress
ReferenceWBPaper00001053