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| WBPicture0000011600 | Description | Figure 5. EU30 and MH42 localize to the middle of A-bands in both body wall and pharyngeal muscle from wild-type animals. (A and B) Animals were simultaneously reacted with a polyclonal ct-twitchin and monoclonal MH42, and secondary antibodies of goat ct-rab- bit-fluorescein and goat a-mouse rhodamine as described in Materials and Methods. A portion of body wall muscle is shown, as viewed with the fluorescein channel in A (to show et-twitchin reaction), and the rhodamine channel in B (to show MH42 reaction). The arrows point to the middle of the same muscle A-band. (C and D) Animals were simultaneously reacted with a monoclonal to myosin heavy chain A (ct-myoA) and polyclonal EU30, and appropriate fluor-labeled secondary antibodies. The same portion of body wall muscle is shown, as seen with the rhodamine channel in C (to reveal a-myoA reaction) and as seen with the fluorescein channel in D (to reveal EU30 reaction). (E and F) Frozen cross sections were simultaneously reacted with a monoclonal to pharyngeal muscle-specific myosin heavy chain C (et-myoC; shown in E), polyclonal EU30 (shown in F), and appropriate fluor-labeled secondary antibodies. E and F are from the same cross section of a pharynx as viewed with the rhodamine and fluorescein channels, respectively. In the cross-sections in F and G (below), EU30 and MH42 also react with the four body wall muscle quadrants. (G) Frozen cross section reacted with monoclonal MH42 and goat a-mouse-rhodamine. Bars, 5 um. | |||
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| Name | FigO.jpg | ||||
| Depict | Expr_pattern | Expr1559 | |||
| Anatomy | WBbt:0005451 | ||||
| WBbt:0005813 | |||||
| Cellular_component | GO:0031672 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1083/jcb.132.5.835 | ||
| Journal_URL | TheJournalofCellBiology | ||||
| Publisher_URL | TheRockefellerUniversityPress | ||||
| Reference | WBPaper00002411 |
