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| WBPicture0000011686 | Description | Figure 4. HAM-2 protein is expressed in the HSN nucleus during migration. Fluorescence photomicrographs of wild-type (A- F) and ham-2(mu1) mutant (G-I) embryos that have been stained with DAPI (blue), an UNC-86 antiserum (green) and a HAM-2 antiserum (red). Each panel presents a left lateral view of the embryo, with the developing tail to the left, the head to the right and the end of the tail and the top of the head both oriented upwards. The right sides are out of focus. (A-C) A wild-type embryo at 410 min after first cleavage, in which the HSN has not migrated out of the tail. (A) DAPI staining. The nuclei of the HSN (large arrow) and the ALN / PLM precursor (small arrow) are shown. (B) Anti-UNC-86 staining. Both the HSN (large arrow) and the ALN/PLM precursor (small arrow) express UNC-86. (C) Anti-HAM-2 staining. The HSN nucleus (large arrow) expresses HAM-2; several HAM-2 expressing cells in the head are out of focus. (D-F) A wild-type embryo at 430 min after first cleavage. (D) DAPI staining reveals the position of the HSN nucleus (arrow). The ALN/PLM precursor nucleus is out of the plane of focus. (E) Anti-UNC-86 staining. Shortly after commencing its anteriorly directed migration, the HSN begins expressing UNC-86 (arrow; Finney and Ruvkun 1990). (F) Anti-HAM-2 staining. During its migration, the HSN also ex- presses HAM-2 (arrow). (G-I) A ham-2(mu1) mutant embryo at 430 min after first cleavage. (G) DAPI staining reveals the positions of the HSN (large arrow) and the ALN/PLM precursor (small arrow) nuclei. (H) Anti-UNC-86 staining. The HSN (large arrow) and ALN/PLM precursor (small arrow) show normal ex- pression of UNC-86. (I) Anti-HAM-2 staining. Although expression of HAM-2 in the head (mostly out of focus) and in the hypodermis (at an earlier stage not shown here) are normal, the HSN does not express HAM-2 in ham-2(mu1) embryos. The HSNs also lack HAM-2 expression in ham2(n1332) animals (not shown). Scale bar, 5 um. | |||
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| Name | FigA.jpg | ||||
| Depict | Expr_pattern | Expr670 | |||
| Anatomy | WBbt:0004757 | ||||
| WBbt:0004758 | |||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1101/gad.13.4.472 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00003422 |
