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| WBPicture0000011824 | Description | Fig. 2. HRP-2 is expressed in nuclei of all life cycle stages of C. elegans. (A-E) GFP fluorescence in different life cycle stages of transgenic line HRP-2DGFP; (A-C) Upper panel, fluorescence; lower panel, DIC. (A) Uterus from an adult hermaphrodite showing fluorescence in early embryos; (B) embryos at three-fold stage showing large gut cell nuclei; (C) L2 with fluorescence in most tissues particularly bright around the pharynx; (D) diffuse nuclear staining in gut cell from isolated adult intestine; (E) punctate staining in neuronal cell nuclei. (F-H) Immunofluorescence with R64 Ab and prebleed. Panel F shows colocalisation of HRP-2 and DAPI staining in tail region of freeze-cracked and fixed L2 stained with DAPI (top), 1:1000 R64 Ab (middle) and merge (bottom). R64 Ab was detected with antirabbit Alexa 488 at 1:300 dilution. Panel G shows HRP-2 R64 reactivity with gut and gonad dissected from adult hermaphrodite. R64 Ab (upper) and DAPI (lower); (H) similar specimen stained with 1:1000 prebleed (upper) and DAPI (lower). | |||
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| Name | Fig1.jpg | ||||
| Depict | Expr_pattern | Expr3044 | |||
| Anatomy | WBbt:0003679 | ||||
| WBbt:0003681 | |||||
| WBbt:0005772 | |||||
| WBbt:0006760 | |||||
| Cellular_component | GO:0005634 | ||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1016/j.yexcr.2004.04.043 | ||
| Journal_URL | ExperimentalCellResearch | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00024309 |
