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WBPicture0000012728 | Description | Fig. 1. Expression and localization of FIGL-1 and the sterile phenotype of FIGL-1 depletion. (A) FLAG-epitope tagged C. elegans FIGL-1 was overexpressed in HEK293 cells. Transfected cells were stained with DAPI (blue) and immunostained with anti-FLAG (red) and anti-a-tubulin (green) antibodies. Merged images are also shown. (B) A C. elegans gonad from the early L4 stage was dissected. Fixed gonad was stained with DAPI (blue) and immunostained with an anti-FIGL-1 antibody (orange). Merged images are also shown. (C) FIGL-1 was detected with an anti-FIGL-1 antibody at several growth stages in the C. elegans wild-type N2 strain. Ten micrograms of lysates were used for each sample. (D) Depletion of FIGL-1 resulted in defective gonad formation and the sterile phenotype. Feeding RNAi method was employed to deplete FIGL-1. Gonads of progeny were dissected, stained with DAPI, and observed. Arrows indicate normal gonads. Arrowheads indicate defective gonads. | |||
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Name | FigA.jpg | ||||
Depict | Expr_pattern | Expr10050 | |||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2012 | ||||
Article_URL | DOI | id | 10.1016/j.jsb.2012.04.022 | ||
Journal_URL | JournalofStructuralBiology | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00041090 |