WormBase Tree Display for Picture: WBPicture0000012728

WBPicture0000012728 Description: Fig. 1. Expression and localization of FIGL-1 and the sterile phenotype of FIGL-1 depletion. (A) FLAG-epitope tagged C. elegans FIGL-1 was overexpressed in HEK293 cells. Transfected cells were stained with DAPI (blue) and immunostained with anti-FLAG (red) and anti-a-tubulin (green) antibodies. Merged images are also shown. (B) A C. elegans gonad from the early L4 stage was dissected. Fixed gonad was stained with DAPI (blue) and immunostained with an anti-FIGL-1 antibody (orange). Merged images are also shown. (C) FIGL-1 was detected with an anti-FIGL-1 antibody at several growth stages in the C. elegans wild-type N2 strain. Ten micrograms of lysates were used for each sample. (D) Depletion of FIGL-1 resulted in defective gonad formation and the sterile phenotype. Feeding RNAi method was employed to deplete FIGL-1. Gonads of progeny were dissected, stained with DAPI, and observed. Arrows indicate normal gonads. Arrowheads indicate defective gonads.
  Name: FigA.jpg
  Depict: Expr_pattern: Expr10050
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2012
    Article_URL: DOI id 10.1016/j.jsb.2012.04.022
    Journal_URL: JournalofStructuralBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00041090