WormBase Tree Display for Picture: WBPicture0000012745

WBPicture0000012745 Description: Figure S2. nrf-5 Is Expressed in Body Wall Muscle Cells, Related to Figure 2 (A-D''') DIC and fluorescent images of wild-type embryos (A-A''', D-D''') or larvae (B-C''') transgenic for Pnrf-5GFP and Pges-1mCHERRRY (A-C''') or Pttr-52mCHERRY (D-D'''). The GFP expression driven by the nrf-5 promoter is distinct from that of mCHERRY controlled by the ges-1 and ttr-52 promoters which drive gene expression specifically in intestine cells. (E-F') DIC and fluorescent images of wild-type animals expressing ssGFP::mNRF-5 driven by the nrf-5 promoter. Diffuse GFP signal was observed throughout the worm. GFP labeling was also found on the basal surface of pharyngeal cells (E, E', arrowheads) and basal and lateral membranes of the intestine (F, F', arrowheads). (G-H''') DIC and fluorescent images of wild-type animals transgenic for both Pnrf-5GFP::mNRF-5 and Pmyo-3mCHERRY (G-G''') or both Pnrf-5GFP::mNRF-5 and Pvha-6mCHERRY (G-G'''). Non-secretory GFP::mNRF-5 was seen in body wall muscle cells, overlapping with Pmyo-3mCHERRY, but not in intestine cells, which are marked by Pvha-6mCHERRY, except for some GFP signal in gut granules (arrowheads). Scale bars in all panels represent 5 um.
  Name: FigS2.jpg
  Depict: Expr_pattern: Expr10098
    Anatomy: WBbt:0005460
      WBbt:0005772
      WBbt:0005813
    Cellular_component: GO:0016323
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2012
    Article_URL: DOI id 10.1016/j.cub.2012.06.004
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00041220