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WBPicture0000012810DescriptionFigure 4. The expression and localization of GLO-2. (A-D) glo-2::gfp was expressed under the control of the operon internal promoter immediately upstream of glo-2. (A-B) GLO-2::GFP expression was first detected in intestinal precursors (marked with asterisks) at the E4 stage of embryogenesis. (C-D) At the 1.5-fold stage, GLO-2::GFP was expressed in intestinal cells (between black arrowheads) and was localized to the cytoplasm. (E-F) Wild-type and glo-2(zu455) pretzel-stage embryos stained with anti-GLO-2 antibodies. glo-2(zu455) embryos lacked staining of hypodermal organelles by GLO-2 antibodies (white arrows) however they retained staining of P-granules (black arrows). GLO-2 antibodies did not stain prominent organelles within intestinal cells (between black arrowheads). Single focal planes are shown in A-D and maximum intensity projections of 1.25 micron Z-stacks are shown in E-F.
NameFigD.jpg
DepictExpr_patternExpr10568
AnatomyWBbt:0005772
Cellular_componentGO:0005737
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2012
Article_URLDOIid10.1371/journal.pone.0043043
Journal_URLPLoSOne
Publisher_URLPLoS
ReferenceWBPaper00041456