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WBPicture0000013008DescriptionFigure S1 Expression pattern of sir-2.4 in C. elegans. Transgenic lines expressing a SIR-2.4 translational GFP fusion (sir-2.4p::sir-2.4::gfp) were utilized to analyze the expression pattern of sir-2.4 in C. elegans. Images of (a-b) L3 or (c-e) late L4/young adult stage transgenic animals (EQ137) expressing GFP protein under control of the sir-2.4 promoter. sir-2.4 is highly expressed in a subset of head and tail neurons beginning at early larval stage, indicated by white arrows. High expression of sir-2.4 is also found in spermathecaluterine valve (sp-ut valve) cells beginning at L4 larval stage, indicated by white arrows. The yellow arrow indicates the nuclear accumulation of SIR-2.4::GFP fusions in these cells. It is worth noting that very weak expression of sir-2.4 is found ubiquitously in most tissues, although it is difficult to capture in these images.
NameFigS1.jpg
DepictExpr_patternExpr10562
AnatomyWBbt:0006751
WBbt:0006759
WBbt:0008217
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2012
Article_URLDOIid10.1371/journal.pgen.1002948
Journal_URLPLoSGenetics
Publisher_URLPLoS
ReferenceWBPaper00041610