WormBase Tree Display for Picture: WBPicture0000013154

WBPicture0000013154 Description: (D) Wild-type germ lines show nhr-114 mRNA expression (purple staining) throughout the germ line. nhr-114(gk849) germ lines serve as a negative control and show no nhr-114 mRNA staining. Asterisk indicates the position of the distal end. (E) Section of extruded intestines. From left to right, DIC microscopy image of intact animal focusing on the most anterior part of the intestine. pv: pharyngeal valve (bracket), i: intestine (dotted line). In wild-type nhr-114 mRNA (dark purple) enriches to the nucleoplasm (arrows). nhr- 114(gk849) intestines serve as a negative control and show no nhr-114 mRNA in nuclei. Right images are longer incubation times of different intestines. (F-H) Characterization of intestinal NHR-114 transgenic protein. (F) Immunostaining of extruded intestines of the EV441 strain (n>20 animals). anti-FLAG detects FLAG::NHR-114 in intestinal cells DAPI visualizes nuclei. Anterior (top); posterior (bottom). antianillin (ANI-2) outlines apical membranes. Scale bar 50 um. (G) Nuclear enrichment of FLAG::NHR-114 in intestinal cells. DAPI-positive bacterial remnants outline intestinal lumen. Scale bar 50 um. (H) Anti-FLAG and anti-tubulin immunoblot. FLAG::NHR-114 protein levels remain unchanged upon nhr-114 RNAi knockdown. Lanes 3 and 4 represent two independent experiments. Effectiveness of RNAi knockdown of endogenous nhr-114 was confirmed by the appearance of steriles in non-transgenic siblings.
  Name: FigS1_A.jpg
  Crop: Cropped_from: WBPicture0000013153
  Depict: Expr_pattern: Expr10759
    Anatomy: WBbt:0005772
      WBbt:0005784
    Cellular_component: GO:0005654
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2013
    Article_URL: DOI id 10.1016/j.cub.2013.02.034
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00042194