WormBase Tree Display for Picture: WBPicture0000013343

WBPicture0000013343 Description: Figure 4. Localization of tetraThymosin-beta in C. elegans embryo, larvae, and adults. (A) (a-e) Immunolocalization of tetraThymosin-beta (left) and actin (middle) at different phases of embryonic development (scale bar, 10 um). In the right panel, colocalization is visualized (red, tetraThymosin-beta; green, actin; blue, DAPI staining). Arrows and stars indicate the tetraThymosin-beta staining at cell-cell contacts and at the site of the nerve ring, respectively. (B) Localization of tetra- Thymosin-beta in WT young adults; anti-tetraThymosin-beta antibody (a and b) and DAPI for DNA (c and d); scale bar, 50 um. (e and f) Merged images of tetraThymosin-beta (red) and DNA (blue). The entire worm is shown in a, c, and e; the head region is enlarged in b, d, and f. TetraThymosin-beta is diffusely detected in the entire body (a) and enriched in the spermatheca (a, Sp), the lumen of the intestine (a, arrow), and the posterior bulb of the pharynx (b, arrowhead). Only a part of the intestine was stained because of incomplete penetration of the antibody. Adult nerve ring was identified as a region where DAPI staining was weak (d, arrow), but it was not stained by anti-tetraThymosin-beta antibody (f, arrow).
  Name: FigD.jpg
  Depict: Expr_pattern: Expr12265
    Anatomy: WBbt:0003732
      WBbt:0005319
      WBbt:0005772
      WBbt:0006749
    Cellular_component: GO:0005911
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1091/mbc.E04-03-0225
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00023907