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WBPicture0000013410DescriptionFigure 3 (A) Schematic of sma-1 gene, indicating the newly isolated nonsense mutation, iv38, and the structure of the sma-1::GFP reporter; a and b designations indicate start positions of each of two sma-1 isoforms. (B) Expression of sma-1::GFP in the posterior pharyngeal bulb. Expression is visible in the myoepithelial cells but is absent from pharyngeal glands (arrowheads). (C-E) Nuclear sma-1::GFP::HIS2B is absent from gland cells; dorsal view of posterior pharyngeal bulb (outlined). (C) sma- 1::GFP::HIS2B is evident in nuclei of pharyngeal muscles and marginal cells; arrowheads indicate weak GFP signal from out-of-plane pm5 pharyngeal muscle nuclei. (D) phat-1::wCherry in g1A glands, with dotted circles showing position of pm5 nuclear signal; no nuclear GFP is evident within the gland cells. (E) Merge of images from C and D, with pm5 nuclei outlined. (F and G) Gland morphology in sma-1(e30) mutants resembles that seen in sma-1(iv38) mutants.
NameFig.3.jpg
DepictExpr_patternExpr12323
AnatomyWBbt:0003673
WBbt:0003732
WBbt:0005451
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2011
Article_URLDOIid10.1534/genetics.111.132449
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00040002