WormBase Tree Display for Picture: WBPicture0000013472

WBPicture0000013472 Description: Figure 3. mir-80 expression is generally high in the presence of food, but low when food is lacking. 3A. Examples of expression of extrachromosomal bzEx207 [Pmir-80LmCherry] line grown in the presence of unlimited E. coli. Note that this transgenic line, typical of 4 lines that have the long mir-80 promoter region, exhibits substantial reporter expression in the first two cells of the intestine (indicated by white+sign) and in the posterior intestine (white bracket). Lower level expression is evident in several other tissues. Animals are adult day 6, but we find no bleed through of signals using red/green filter sets (Fig. S4B) so age pigments do not confound this analysis. 3B. Examples of expression of the bzEx207 [Pmir-80LmCherry] line grown in the presence of unlimited E. coli until young adulthood and then switched to no food for 48 hours. 6 day old adults are aligned with anterior to the left, posterior gut region indicated by white bracket. Most posterior gut fluorescence is markedly diminished, although expression in the anterior two intestinal cells, the central egg laying muscles, and the very posterior gut remains high. 3C. Quantitation of fluorescence signals for a mir-80 promoter fusion reporter line in food vs. food limitation. Fluorescence of overall bzEx207[Pmir-80LmCherry] line expression after 48 hrs on no-food plates. Food limitation in these studies was by dietary deprivation [28], but food dilution on solid NGM media [4] and food dilution in liquid media [4] induced similar changes in these lines (Fig. S4). Graph represents spectrofluorimeter measurements of fluorescence levels (whole body) for at least 50 animals per DR regimen. Pairwise comparisons were made using Two-tailed Students'T-test. *** - p,0.0005. Same exposure times were used for complementary panels. 3D. Analysis of food-regulated expression of pmir-80LmCherry expression along the nematode body implicates posterior intestinal regions as a major site of regulation. We compared pmir-80LmCherry signals in transgenic ZB3042 grown either in the presence of food (blue) or switched to no food for 24 hrs (red) (measured at day 4, n = 39). We used the ImageJ program to create a 25 pixel segmented line covering the animal and measured mean fluorescence intensity along the body, dividing the length into 12 equal bins and plotting the mean fluorescence intensity at each point. Representative animals are depicted above with the approximate body positions indicated (H = head, P = pharynx, V = vulva, T = tail). Note that although food regulation is apparent in most of the body, food-regulated expression changes in the regions of the mid- and posterior intestine are most dramatic. Error bars indicate standard error for each bin measurement.
  Name: FigC.jpg
  Depict: Expr_pattern: Expr11257
    Anatomy: WBbt:0005772
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
    Publication_year: 2013
    Article_URL: DOI id 10.1371/journal.pgen.1003737
    Journal_URL: PLoSGenetics
    Publisher_URL: PLoS
  Reference: WBPaper00044166