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WBPicture0000013902DescriptionFigure S5, related to Figure 2. lite-1 expression rescues the defects of lite-1 mutants, andlite-1 is expressed in 29 cells.(A) The lite-1 gene, showing the design of several lite-1 transgenes. The lite-1 gene model wassemi-automatically constructed using the Exon-Intron Graphic Maker (available at http://www.wormweb.org).(B) The lite-1 genomic locus rescued the pumping inhibition defect of lite-1(ce314) mutants.(C) The lite-1prom::lite-1::gfp transgene rescued the pumping inhibition defect of lite-1 mutants.(D) The lite-1prom::gfp::lite-1 transgene rescued the pumping inhibition defect of lite-1 mutants.(E) Quantification of acute response latency.(F) Quantification of acute response amplitude.(G) Quantification of recovery response amplitude.(H) Strains carrying all three lite-1 transgenes were rescued for the light avoidance defect of lite1mutants. Body bends were scored during 10 s of light exposure.(I) Expression pattern of the transcriptional fusion (lite-1prom::gfp) in the adult head and tail. Noexpression was observed in the midbody.(J) Dorsal view of the transcriptional fusion expression pattern in the adult head.(K) Expression pattern of the C-terminal translational fusion (lite-1prom::lite-1::gfp) in the adulthead and tail. No expression was observed in the midbody.For behavioral assays, n = 60 worms. Shading around traces and error bars indicate s.e.m. * p <0.05, ** p < 0.01, *** p < 0.001, ns = not significant, t-test, compared to the wild type or to thestrain indicated. d = dorsal, a = anterior, r = right. Scale bars, 8 um.
NameFigS5.jpg
CropCrop_pictureWBPicture0000013903
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year2015
Article_URLDOIid10.1016/j.neuron.2014.12.061
Journal_URLNeuron
Publisher_URLElsevier
ReferenceWBPaper00046411