WormBase Tree Display for Picture: WBPicture0000013977

WBPicture0000013977 Description: Figure 3. Temperature Upregulates acdh-11, Causing Downregulation of fat-7 Expression(A) Representative Nomarski and GFP fluorescence micrographs of wild-type transgenic animals with nIs677[Pacdh-11::GFP] (left), the expression of which isupregulated by high temperature at 25C. A high-magnification view of another animal (right) shows GFP predominantly in intestinal cells (arrows). Scale bars, 100 um.(B) qPCR results showing that endogenous acdh-11 is transcriptionally upregulated at 25C. p < 0.01 (n = 4 for each genotype).(C) RNA-seq quantification of the expression levels at 15C, 20C, and 25C (normalized to levels at 20C) of genes encoding all seven C. elegans lipid desaturases(fat-1 to fat-7). Arrow indicates downregulation of fat-7 expression by temperature. FPKM, fragments per kilobase of exon per million fragments mapped.(D) qPCR quantification showing fat-7 expression levels in wild-type animals and acdh-11 mutants. p < 0.01 (n = 4 for each genotype).(E) Representative Nomarski and GFP fluorescence micrographs of wild-type nIs590 transgenic animals showing that RNAi against acdh-11 induces FAT-7::GFPexpression at 25C. Scale bars, 100 um.(F) RNAi against all acdh gene family members showing that acdh-11 was specifically required for downregulating FAT-7 abundance at 25C. p < 0.01 (n = 100 foreach of four independent experiments).
  Name: Fig3_A.jpg
  Crop: Cropped_from: WBPicture0000013976
  Depict: Expr_pattern: Expr12282
    Anatomy: WBbt:0005772
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2015
    Article_URL: DOI id 10.1016/j.cell.2015.04.026
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00046830