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| WBPicture0000014153 | Description | (A) GFP expression of oyIs35 animals carrying an integrated lim-4p::gfp reporter is overlapped with expression of the flp-12p::mCherry reporter (the SMB marker) but not with expression of the ceh-17p::dsRed reporter (the SIA marker). Images are derived from z-stacks of confocal microscopy images while images in the upper-left boxed regions are single focal plane confocal microscopy images. Anterior is to the left. Scale bar: 50 um. (B) In lim-4 mutants, expression of the lim-4p::gfp reporter is abolished in the cell bodies and processes of the SMB neurons in adult but not L1 larval stage animals. GFP expression of the lim-4p::gfp reporter is shown in wild-type(left column) or lim-4(lsk5) mutant (right column) animals at L1 larval (top) or adult stage (bottom). Note that the lim-4p::gfp reporter is not expressed in the AWB neurons of lim-4 mutants [14]. Images are derived from z-stacks of confocal microscopy images. Quantitative analysis of these phenotypes is shown in Table 1. Anterior is to the left. Scale bar: 20 um. (C) Percentage of animals of the indicated genotypes expressing stably integrated flp-12p:gfp reporter (ynIs25) is shown. Strong, weak or off expression is defined as GFP expression observed at 400x magnification in both cell bodies and processes, in only cell bodies, or not observed either in cell bodies or processes, respectively. Heat shocks were treated to L4 larval stage animals at 33C twice for 30 minutes and after 14 hours, phenotypes were analyzed. Over two independent transgenic lines were tested. n=50 for each. | |||
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| Name | Fig4_A.jpg | ||||
| Crop | Cropped_from | WBPicture0000014152 | |||
| Depict | Expr_pattern | Expr12499 | |||
| Anatomy | WBbt:0004973 | ||||
| WBbt:0004974 | |||||
| WBbt:0004975 | |||||
| WBbt:0004976 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2015 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1005480 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00048472 |
