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WBPicture0000014191DescriptionFigure 4. The exocyst regulates alae formation independently of RAL-1. (A) Quantitative electron microscopy analysis reveals that the MVB density issimilar in sec-5(pk2358) and sec-8(ok2187) null mutants and WT controls. MVB density is increased after sec-8(RNAi) but not sec-15(RNAi) comparedwith control(RNAi) (P values, Mann-Whitney test). (B) Example of an MVB attached to the apical plasma membrane in sec-5(pk2358) (enlarged in B').(C) Small vesicles (<100 nm diameter; black arrows) accumulating under the plasma membrane in sec-8(ok2187) mutants. (D and E) The exocyst subunitsSEC-8 (D) and SEC-15 (E) do not colocalize with VHA-5 in the epidermis. The scale bars in D apply to E. APM, apical plasma membrane; Cu, cuticle; Cy,cytoplasm; MVBm, MVB outer membrane
NameFigD_A.jpg
CropCropped_fromWBPicture0000014190
DepictExpr_patternExpr12502
Expr12503
AnatomyWBbt:0005733
Cellular_componentGO:0000145
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>.
Publication_year2015
Article_URLDOIid10.1083/jcb.201504136
Journal_URLTheJournalofCellBiology
Publisher_URLTheRockefellerUniversityPress
ReferenceWBPaper00048680