WormBase Tree Display for Picture: WBPicture0000014268

WBPicture0000014268 Description: Fig 1. C. elegans tmem-218 encodes a novel transition zone protein and genetically interacts with nphp-4 but not mks-2, consistent with it beingpart of the Meckel syndrome (MKS) module. (A) Amino acid sequence alignment of C. elegans TMEM-218 and its Homo sapiens orthologue. Bothproteins are predicted to encode proteins with three transmembrane domains (TM1-3), with the predicted topology shown schematically. (B) C. eleganstmem-218 (T23E7.5) is expressed specifically in ciliated sensory neurons and its encoded product (TMEM-218) tagged with green fluorescent protein (GFP;green) localises to the ciliary transition zone (TZ) at amphid (head) and phasmid (tail) cilia found at the distal ends of dendrites. The tdTomato-tagged intraflagellar transport (IFT) protein XBX-1 (red) marks basal bodies (BB) and ciliary axonemes. Scale bar, 4 um. (C) The tmem-218(nx114) mutant allele used in this study, generated using transposon-mediated mutagenesis, represents a complete and specific deletion of the tmem-218 gene. (D) Dye-filling assay schematic shows the fluorescent dye DiI (red) penetrating sensory neurons via structurally intact, environmentally exposed ciliated endings. Dye-filling of the tmem-218 and nphp-4 strains is normal (+++) or slightly reduced (++), respectively, whereas dye-filling of the tmem-218;nphp-4 double mutant is mostly disrupted (+). A lack of genetic (synthetic) interaction between tmem-218 and mks-2 is also consistent with TMEM-218 belonging to the MKS module (as depicted schematically). Scale bar, 40 um.
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  Crop: Crop_picture: WBPicture0000014269
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
    Publication_year: 2016
    Article_URL: DOI id 10.1371/journal.pbio.1002416
    Journal_URL: PLoSBiology
    Publisher_URL: PLoS
  Reference: WBPaper00049372