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| WBPicture0000014462 | Description | Fig 5. DAF-28::mCherry is expressed in punctate pattern in axons of head neurons. A,B. Single plane images of same animal expressing pdaf-28::GFP transcriptional reporter (wwEx85) and DAF-28::mCherry protein (low expression transgene). pdaf-28::GFP expression is seen in multiple neuronal cell bodies (c.b.), in axons (ax) and dendrites (d), in hypodermis (hyp), and pharynx (ph). C,D. DAF-28::mCherry protein is bright in coelomocytes (cc) of mid-L1 larva relative to its intensity in neuronal cell bodies (c.b.), indicating its efficient secretion. DAF-28::GFP accumulates in cell bodies. Both can be seen in a regular punctate pattern in axonal region. p.i. indicates posterior intestine. D, D' and D'' are enlarged areas boxed in C. D' and D'' are green and red channels of the same image. E. DAF-28::mCherry protein rescues persistent dauer entry of the 4xDel strain (missing four pro-growth IGF-like proteins, including DAF-28, INS-4 and INS-6). F. DAF-28::mCherry protein (red) localizes in a regular pattern in large puncta adjacent to the ASI axon, as well as in pharyngeal and hypodermal tissues. Green, pdaf-7::GFP;rol-6 array used to visualize ASI neuron (ASI::GFP). Orientationof the ASI neurons is shown in panel H. G. Inactivation of the C. elegans homologue of axonal kinesin KIF1A, unc-104, eliminated axon-adjacent puncta of DAF-28::mCherry. Accumulation of mCherry-positive vesicles,presumably representing the DAF-28::mCherry secreted from the cell body, can be seen instead. H. Schematic representation of orientation of the ASI neuron relative to the pharynx. Anterior is to the left, dorsal up. The ASI cell body, dendrite, and the proximal and synapse-rich distal axonal regions are indicated. All confocal images of ASI neurons follow this orientation. | |||
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| Name | FigR.jpg | ||||
| Depict | Expr_pattern | Expr13308 | |||
| Anatomy | WBbt:0003681 | ||||
| WBbt:0005733 | |||||
| WBbt:0005751 | |||||
| WBbt:0006751 | |||||
| WBbt:0006759 | |||||
| Cellular_component | GO:0005576 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1371/journal.pgen.1006450 | ||
| Journal_URL | PLoSGenetics | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00050499 |
