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| WBPicture0000014667 | Description | Fig 3. Quantification of lin-22 expression in the seam. (A) Transgenic animals carrying transcriptionalreporters consisting of various fragments of upstream of lin-22 sequences fused to GFP. From top to bottom:full lin-22 endogenous promoter, distal lin-22 promoter region that is deleted in lin-22(icb38), proximal lin-22promoter present in lin-22(icb38), distal lin-22 promoter with deleted CR1, and CR1 only driving expression ofGFP. White arrows indicate expression in the seam cells; white arrowheads expression in the hypodermis andgreen arrowheads expression in intestinal cells. (B) Quantification of expression pattern for eachtranscriptional reporter (n=35 animals). (C) Representative smFISH images showing lin-22 expression(black spots correspond to mRNAs and seam cells are labelled in green due to scm::GFP expression) in wildtype V cells after the symmetric L2 division (top), the L2 asymmetric division (middle), and late after the L2asymmetric division (bottom). (D) Quantification of lin-22 spots per seam cell in wild-type and lin-22(icb38)animals at the late L1 stage (n 10 cells per genotype). (E) Quantification of lin-22 spots in wild-type, lin-22(ot267), lin-22(ot269), and lin-22(icb49) mutants in pools of H cells and V cells at the late L1 stage (n 41).(F-G) Comparison of number of lin-22 spots between wild-type and the elt-1(ku491) mutant (F) or the egl-18(ok290) mutant, (G) both at the late L1 stage in pools of H and V cells (n 49). Black stars show statisticallysignificant changes in the mean with a t test or one-way ANOVA as follows: * P < 0.05, ** P < 0.01,*** P < 0.001, **** P < 0.0001. Reference samples for comparisons in E, F, G are the control samplesdepicted in black. Scale bars in A and C are 100 μm and 10 μm, respectively. Error bars show mean +- SEM(D, F, G) or mean ± SD (E). Numerical data used for Fig 3B, D, E, F, G can be found in S2 Data. CR1,conserved region 1; GFP, green fluorescent protein; L1, first larval stage; L2, second larval stage; smFISH,single molecule fluorescent in situ hybridization. | |||
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| Name | FigT.jpg | ||||
| Crop | Crop_picture | WBPicture0000014670 | |||
| Depict | Expr_pattern | Expr13573 | |||
| Anatomy (14) | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2017 | ||||
| Article_URL | DOI | id | 10.1371/journal.pbio.2002429 | ||
| Journal_URL | PLoSBiology | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00053295 |
