WormBase Tree Display for Picture: WBPicture0000014835

WBPicture0000014835 Description: Figure S7. EGL-20 Is Expressed in Neurons in C. elegans, Related to Figure 7(A) Representative photomicrographs of animals expressing neuronal EGL-20; hsp-6p::gfp in WT, ser-4(ok152), ser-7(tm1325), ser-1(ok345), or ser-1(ok345); ser7(tm1325) double mutants background.(B) Representative photomicrographs of animals expressing neuronal EGL-20; dve-1p::dve-1::gfp in WT or mod-1(ok103) background.(C) Representative photomicrographs of egl-20p::egl-20::mcherry in WT, vps-35(ok1880), or mig-14(ga62) background.(D) Quantitative PCR of egl-20 mRNA level. Synchronized Day 1 adult animals of WT, rgef-1p::Q40::yfp were collected for qPCR. ns denotes p > 0.05 via t test.Error bar indicates the SEM from six biological replicates.(E) Immunoblots of mCherry and YFP expression in egl-20p::egl-20::mcherry animals in the presence or absence of neuronal Q40. Anti-tubulin serves as a loadingcontrol.(F) Quantitative PCR of Wnt target genes mRNA level. Synchronized Day 1 adult animals of WT and rgef-1p::Q40::yfp were collected for qPCR. *p < 0.05,**p < 0.01, ns denotes p > 0.05 via t test. Error bars indicates the SEM from six biological replicates.(G) Representative of three center images of confocal photomicrographs of animals expressing egl-20p::mcherry or egl-20p::egl-20p::mCherry in combinationwith rgef-1p::Q40::yfp. Scale bar represents 50 um.(H) Representative photomicrographs of animals expressing egl-20p::egl-20p::mCherry in combination with rgef-1p::yfp or rgef-1p::Q40::yfp. Scale bar represents 50 um.
  Name: FigS7.jpg
  Crop: Crop_picture: WBPicture0000014836
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2018
    Article_URL: DOI id 10.1016/j.cell.2018.06.029
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00054967