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WBPicture0000015235DescriptionFigure 1. Expression of ODD-2::GFP in the intestine, the tail region and sheath cells:Images were taken of JR2005 or ACG10 with an Olympus BX61 confocal microscope. All images show a single plane of view, except D and F which depict the GFP as a Z projection. In each set of three images, the left image is the fluorescent image, the middle is the brightfield, and the right image is the merge. Worms were immobilized with levamisole; in D-F, they were immobilized with levamisole and Nemametrix Nemagel. A-C) Images of a JR2005 worm at 40x magnification showing expression in the posterior intestinal cells and cells posterior to the intestine. D-F) Images of a JR2005 worm at 100x magnification. Possible rectal gland expression is indicated by white arrows. The GFP is a Max Intensity Z projection of 11 slices. The brightfield image is the middle slice. G-I) Images of an ACG10 worm at 40x magnification showing that BUS-1 expression (red) is largely posterior to the ODD-2 expression (green). Insets show enlarged versions of the end of the tail region. J-L) Images taken at 40x show expression of ODD-2 in the vicinity of the germline. Arrows indicate the cell outside of the intestine that is expressing ODD-2 (likely a sheath cell). M-O) Images show an extruded germline at 40x magnification with two likely sheath cells expressing ODD-2 indicated by arrows.
Name2743977d7fd0954b6f5fd717a1bb025c.jpg
DepictExpr_patternExpr16349
AnatomyWBbt:0005772
WBbt:0005799
WBbt:0005828
AcknowledgmentTemplateReprinted from <Journal_URL>
Publication_year2023
Article_URLDOIid10.17912/micropub.biology.000967
Journal_URLmicroPublicationBiology
Publisher_URLMicropublication
ReferenceWBPaper00065912