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WormBase Tree Display for RNAi: WBRNAi00081457

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Name Class

WBRNAi00081457HomolHomol_homolC28G1:RNAi
Sequence_infoPCR_productsjj_C28G1.3
ExperimentLaboratoryLJ
Date08 Jun 2009 00:00:00
Genotypewts-1(ok753)/hT2;Ex[gfp::act-5, pRF4]
TreatmentL3 and L4 transgenic worms were grown to the adult stage on the RNAi medium. When they had reached the adult stage, they were transferred to new RNAi medium and allowed to lay eggs for 12 to 16 hours; then the adults were removed. After 3 days at 20C, mutant worms that showed no pharyngeal GFP signal were examined for GFP::ACT-5 localization.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneC28G1.3Inferred_automaticallyRNAi_primary
GeneWBGene00016188Inferred_automaticallyRNAi_primary
TranscriptC28G1.3.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000051539
ReferenceWBPaper00034726
PhenotypeWBPhenotype:0000679RemarkRNAi of sec-15 significantly suppressed the severe intracellular vesicle-like GFP::ACT-5 localization phenotype in wts-1 mutant animals
MethodRNAi