WormBase Tree Display for RNAi: WBRNAi00081462
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| WBRNAi00081462 | Homol | Homol_homol | Y105E8B:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | atgctttcaaatgaggatggattattccagacggacgccttttctccatcggattacatccgggaccgcctgaaaggggtgaaaatcggtgatgagacacgaaaacttcgacatcttcgttcagaaatgggcgctctgaaccatgcttcacaggagacactgaaaaataacgtttttcgaaattatcagcaatttattgatgcttcgaaagagatttctcatttggagcgagaaatctaccaattaacacattccctaatcgagcaaaaacaagtaatcgagaatttaataatgatgactggagatgataaatcatcaattcatacagtatcaagtcattctacaaccacctcgaccactaatccaattcaaattttaatgcagaaaatggagggaatcggcgggattttaaataatatgtcaacttcggaaaaagtaattttgtacggggaaatggagcaattcgacgtggaaacaaagaagccagtgcagaagtgtatgctgatattattgtctcatcgattgattattggaaatattaatgctgctggaaaatatgtaatggaatcaaccctctcactaaactcagttgccccagtcaacgttaaagatcgtgaatcaggcgccgccaacgctggaaaagtgctgaaactcttgatattccccgaatcccgttgttatttgtgtgagagtgcaagaattcgaaccctttggtttgacgagttggaacaggcgaaacgagagattctacataagggaagtctcgtgagacaggcaactattcgaggaaaacggcaaactgtgaaagaacggaaggccgctgcatcgtcggctattgaggaaaaaccatcttttctagaggaagaggaggatgtcggggatgaaaatgaagagagaaacgacaatgacatttcctggttatccgaacttccggcagaactggacgattgcattgctcatcgagaccttgatcaagctgttgagcttattcacgaatggaaacagtgcccgacgaaagatgctgtaattgatattcagttgcaaatgagagagaatacgattgtccaactgctcagcgacgatgtaagccggcccggcgcagtacacggaggcccgagagccatgaaaaaagcccgaactcttctaacccaattaggccgtggaacctacgcaacagatgtctatttaagacggagaagtgcaatgcaacgaagtgcaatgcgtgatgtaacagtatcagaagaaccaatatcatatgtaaagcaactatgtacattatatggaaatgcagtatctgatattgcaaatgaattccgatcaatgcctcaatactattgtcaagtacttcaatggtgttcttatgaattaagtactctattgaatttgatcagaagacatgttattgaagttgcacctgcaattgctgtaattgcatatacgtggaagaatgtaatgcatacaatggaagatctcactgcaattggtgttcatttgaatttcgaagtcaatcggcttttaagtattcccctcgagaaatctctacaaggaaactttgaaaatatctgtgatgctgtgaaaatgcggatttcggaggaaaaatggaggccctacgtgctggaatcggaatctgcactgaatcgactcgttgaggagctttccgatgttgggattaatgtggaatgggcgatttctgcaaatgatgaatcgagaagctcgttgaatgtttcgcagaatgttgtgcattttgcgaaagttgcacatactctttcgaaggatttggcgccgcttagattatccccaatccaagtgttgtgtgaagtgtatgcagatgaactatggaagatgtatctacaacatttggctgataacatccgagacggcgccgtttttgcgtattccactacttttattctaaccatggtactcccagtgtgcgataaaatcctatacggcgaggaaggcgagctcattcgcctgattgatcaattccccagcctggctccgtacgcagacccatctgatgaagagaagaaggatgatgaagaagttgctcatgtttga | |||
| Experiment | Laboratory | LJ | |||
| Date | 08 Jun 2009 00:00:00 | ||||
| Genotype | wts-1(ok753)/hT2;Ex[gfp::act-5, pRF4] | ||||
| Treatment | L3 and L4 transgenic worms were grown to the adult stage on the RNAi medium. When they had reached the adult stage, they were transferred to new RNAi medium and allowed to lay eggs for 12 to 16 hours; then the adults were removed. After 3 days at 20C, mutant worms that showed no pharyngeal GFP signal were examined for GFP::ACT-5 localization. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | Y105E8B.2b | Inferred_automatically | RNAi_primary | |
| Y105E8B.2a | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00013687 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y105E8B.2b.1 | Inferred_automatically | RNAi_primary | ||
| Y105E8B.2a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000051543 | ||||
| Reference | WBPaper00034726 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | RNAi of exoc-8 significantly suppressed the severe intracellular vesicle-like GFP::ACT-5 localization phenotype in wts-1 mutant animals | ||
| Remark | Authors state that they used full length cDNA as RNAi probe; spliced coding region sequence of gene used for curation. | ||||
| Method | RNAi |
