WormBase Tree Display for RNAi: WBRNAi00083336
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| WBRNAi00083336 | Homol | Homol_homol | Y18D10A:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | caagtgacggagctccagctgcctctgcaggttcttccagagccggaacgccatcccgtaacagtccgttaggccaaatcatccaaaatcaacgtccaggacgtggaggttatcagcaaagtcagttttattttctttcaaatcaactctggatttatgatcatgttttcagacttccaagctaaccgtggatacaacaactaccgcggaggattcgctggcggatacaacaaccaaagagggcacaacaattatggagttcctcgagttaatcaccgcgaaaagctgaagttcgaatcggactttgacttcgagaaggccaatgagaagttccaagaagttctcgtcgacaaccttgagaagctcaacattgaggataaggccgagccagaagttgaggagaagaaggatgctgccttctacgacaagaagacttcattctttgacaacatcagctgtgaatcactggagaaagccgaaggaaagactggacggccagattggaagaaggagagagaaacgaatcaggagacgttcggacacaacgcagtgcgttcgttgaactaccgtcgtggattcggaggacgaggacgtggtggaaaccgcggatatggaggtatagttccactttagaagttacaacgtcttatgctcaattttcaggttacaacaacggataccagcatcaacaccagcaccgtggaggatacaatggaggttatcgtcagaacaacggtggataccgtcgtggtggatatgctccacgtgacaa | |||
| tgtcgaatcaaacaccgtacatcggaagcaagattagtctgatctccaagctcgacatccgttacgagggaatcctctacaccgtcgacaccaacgactctaccatcgcgttggccaaggttcgctcgttcggaaccgagaagcgtccaactgctaatccggtcgccgctcgtgatgatgtctacgagtacatcatcttcaaggccagcgacatcaaggatttgatcgtttgtgatactccgaaggcgagttgccggataatcgagcttcattgatctaaataatgtcttttcagatggccaacattggaggagggcttccatacgatccagccatcatctctgtct | |||||
| ttcgaattccctgaaattgttttctccccacatccccataccccgatattagccgtttgttaatttattatgtaagtttatccattctccccccccccccatcaatgattacccttgtctcttctcaactattggcgtgttataagaaacccgttaattatttagaaatctcgtacgattcaatgcgggccttctgttattttgtaataattattgcgcacccacccaccgacacacatctcccatccaaaaaattcccaatgttttttcttcccccactacaatagtgattggacccttcccccgtttttttaagttctagcttgtgaattttaaagcatgtcgttctgttgtcctcagtttgtcattttaataaaattgcagtgactaaactgtcaaatcgtttgcacctgctctcctatcatcccacaccccgccttttatgattgtcttctaggagatatgaaattgtttttaagatcttctcttagatttgctataatattgcaaacccttcattcttaggtagacaagccctgagaaattgtga | |||||
| Experiment | Laboratory | OD | |||
| Date | 21 Sep 2005 00:00:00 | ||||
| Treatment | L4 hermaphrodites were injected with dsRNA and were incubated at 20 C for 45 h before analysis. | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | Y18D10A.17 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00012484 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y18D10A.17.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00026922 | ||||
| Phenotype | WBPhenotype:0001102 | Remark | Spindle density was reduced compared to controls; at anaphase, spindle poles separated abruptly and prematurely (spindle snapping) | ||
| WBPhenotype:0001129 | |||||
| WBPhenotype:0001343 | |||||
| WBPhenotype:0001403 | Remark | AIR-2 is lost from interzonal microtubules (and remains localized only to chromosomes) during anaphase, as determined by antibody staining; ZEN-4 is lost entirely from spindles, as determined by antibody staining | |||
| WBPhenotype:0001875 | |||||
| Phenotype_not_observed | WBPhenotype:0000679 | Remark | CGH-1 and GFP::PGL-1 localization were unaffected | ||
| GFP::AIR-2 is lost from interzonal microtubules (and remains localized only to chromosomes) during anaphase; GFP::ZEN-4 is lost entirely from spindles | |||||
| Remark | Authors report the use of three different targeting sequences for RNAi of car-1; authors did not specify which was used for this experiment; all three have been used for curation | ||||
| Method | RNAi |
