WormBase Tree Display for RNAi: WBRNAi00084451
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| WBRNAi00084451 | Homol | Homol_homol | K02B12:RNAi | |
|---|---|---|---|---|
| T07A9:RNAi | ||||
| Sequence_info | DNA_text | atgagcgcagatgagccgtctccagaagacgaaaaatatctggaaagtctacgagatttgctgaaaatcagccaagaattcgatgcaagtaacgcaaaacagaatgacgaaccagaaaaaacagcagttgaagtggaatcggctgaaaccagaaccgacgaatctgaaaaatcaatcgatattccacgtgaacagcaacttttaccctccgaacgtgttgagcctttaaaatcaatggtggaaccagaatacgtgaaaaaagtgataaggcaaatggatacaatgactgctgaacagctaaaacaagcgttgatgaagattaaagtgtccacaggcggaaacaagaaaactcttcgaaaacgggttgcacaatattatcgaaaggaaaatgcattattgaatcgaaaaatggagccgaatgccgataaaactgcacgcttttttgactatttaattgcaattgatttcgagtgtacgtgcgtcgagattatatacgattacccacacgaaatcatcgaattaccggcagttttgatcgacgttcgggaaatgaaaattatcagcgagtttcgaacctatgtccgacctgtcagaaatccgaagctcagcgaattttgtatgcagtttaccaaaattgcccaagaaacagtcgatgctgctccatatttccgagaggctcttcaacggctgtacacatggatgcgaaaattcaatctcggacagaaaaattccagattcgcatttgttactgatggacctcacgatatgtggaaattcatgcaatttcaatgccttttatcgaatattcgaatgccccatatgttccgaagttttatcaacatcaaaaagacattcaaagagaaattcaatggacttatcaagggaaatggaaaatccggaattgaaaacatgttagaacgtctggatctgtcgtttgttggaaataaacatagtggattggatgatgcaacgaatattgcagcgattgcgattcaaatgatgaagttgaaaattgagttgagaattaatcagaaatgctcgtataaggagaatcaacggagtgctgcgcgaaaggatgaagagcgagagttagaagacgctgcaaacgtggatcttacatcagtcgatatctctcgtcgtgattttcaactttggatgcgtcgtttaccactgaaactgtcctctgtgacacgacgagagttcataaatgaagagtatttggattgtgattcatgtgatgatttgactgatgataaggtgaaacatctacattcttgtgatatttatgaaattttcgatgaaaaaacttcagctagttttacagattcaaagtgtttgatttgctag | ||
| atgctcataccttcgtcgtcatccataccgtcttctttatctgcttcagcatccgattctgagccatcatcactgaacggaagtggtataagtgatcagaatattctacctaattatcatcttcatcatcatctaattaacgagaacgagatggaggcagccaattatgcacaggttatcaaaccaacatgcgaggcattccaagattggccgcatactccaatgctgtatcaacaacctcaacttcatttcatgcttccacaacacgattgggcatatccacatcttgctcaatctctacctcctccacatcatctaacaccgtccactgcagcagttgctgctgcaacaattgcctcacaaagttcaataatcaatcaaacgtctgtggttacaagtacaccttcgtgccaaatcaaacaggaagttgaacgacctgaaattattcagaggttaatgcctccatggccaccagcttatcaattttcttgtgatgatagtggatcagtgagtggagctggtggaccacatcagccattatctgatatttccgatgatagtgaacagacgtgtccagatgatttggaaggatttgcaaaacaatttaagcagagaagaatcaaattaggatacacacaagcagatgtaggtgtggctctcggaacactctacggaaatatattttcccagacaacaatttgtcgttttgaagcgcttcaactctctttcaagaatatgtgcaaactaaagccacttctattcaaatggctcgaggaagctgattcaacgactggctcgcccaattctacatttgaaaaaatgacagggcaagctggaagaaagagaaagaagagaacaagcattgaggtcaatgtaaaatctcgtcttgaattccatttccagtcgaatcagaagccaaatgcacaagagattacacaagttgccatggagttgcagcttgagaaagaggttgtccgtgtctggttctgcaatcgtcgtcaaaaagagaagcgaatagctccaaatcaatacgatgctccacatccaatggctttaaacaatggatatccaatgactgctgaccttttcccatatcaagccgtcgtcaatcattatactcaacagagcccgagacaacaatag | ||||
| Experiment | Laboratory | CM | ||
| Date | 23 Oct 2009 00:00:00 | |||
| Strain | WBStrain00002259 | |||
| Treatment | Injected nematodes were transferred to plates to recover for 24 h before being transferred to fresh plates to lay offspring for 24 h. Thirty F1 progenies (L2 stage) were scored at 48 h post injection for the presence or absence of GFP transgene expression. Additionally, we scored a minimum of 30 F1 progenies from three sister plates of nematodes carrying each transgene at 72 h post injection for developmental delays and lethality to assess the efficiency of the ceh-6 knock down. | |||
| Delivered_by | Injection | |||
| Inhibits (3) | ||||
| Species | Caenorhabditis elegans | |||
| Reference | WBPaper00035475 | |||
| Phenotype | WBPhenotype:0001278 | Remark | The percentage of animals expressing the vha-13::gfp transgene in the excretory cell was significantly reduced | |
| Remark | Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | |||
| Method | RNAi |
