WormBase Tree Display for RNAi: WBRNAi00085149

WBRNAi00085149 Homol: Homol_homol: ZK1127:RNAi
      T02G5:RNAi
  Sequence_info: PCR_product: sjj_ZK1127.1
  Experiment: Laboratory: YL
    Date: 24 Jan 2011 00:00:00
    Genotype: meg-1(vr10); PGL-1::RFP
    Treatment: Hermaphrodites were raised at 20C until the L4 stage, then moved to RNAi feeding plates seeded with nos-2 RNAi feeding bacteria and maintained at 26C. Animals were allowed to reach adulthood and then transferred to fresh nos-2 RNAi feeding plates every hour to give populations of staged L1 larvae.
    Temperature: 26
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: ZK1127.1 Inferred_automatically: RNAi_primary
      ZK1127.11 Inferred_automatically: RNAi_primary
    Gene: WBGene00003784 Inferred_automatically: RNAi_primary
      WBGene00001872 Inferred_automatically: RNAi_primary
    Transcript: ZK1127.1.1 Inferred_automatically: RNAi_primary
      ZK1127.11.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00038112
  Phenotype: WBPhenotype:0000215 Remark: meg-1(vr10) animals exposed to nos-2 RNAi and grown at 26 degrees C developed gonads devoid of germ cells; PGL-1::RFP expression, a marker of P granules, was mislocalized from the nuclear periphery and dispersed aberrantly into the cytoplasm; 57% of gonads failed to proliferate beyond the first two primordial germ cells, Z2 and Z3.
    WBPhenotype:0000679 Penetrance: Range: 9 71
    WBPhenotype:0000823 Penetrance: Range: 57
    WBPhenotype:0001302 Penetrance: Range: 9 71
  Method: RNAi