WormBase Tree Display for RNAi: WBRNAi00085170
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| WBRNAi00085170 | Homol | Homol_homol | F26H9:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F26H9.6 | |||
| Experiment | Laboratory | HY | |||
| Date | 20 Dec 2010 00:00:00 | ||||
| Genotype | unc-119(ed3) [opt-2::GFP::pgp-1, unc-119(+)] | ||||
| Treatment | For determination of endocytosis after RNAi treatment, PGP-1::GFP animals were synchronized to the L1 stage by hypochlorite treatment and overnight starvation in M9 media at room temperature and then seeded onto RNAi plates and grown to the L4 stage at 20C, before being exposed to OP50-Cry5B for 2 hr at 20C. 1:10 dilutions of rab-5 RNAi were made by combining one part RNAi culture with nine parts pL4440 (empty vector) bacteria | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F26H9.6 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004268 | Inferred_automatically | RNAi_primary | ||
| Transcript | F26H9.6.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00038144 | ||||
| Phenotype | WBPhenotype:0000679 | ||||
| WBPhenotype:0001422 | Remark | RNAi causes significant inhibition of Cry5B-induced endocytic response, as indicated by the PGP-1::GFP transgene | |||
| Method | RNAi |
